Journal: Nanomaterials

Article Title: Salivary Leucocytes as In Vitro Model to Evaluate Nanoparticle-Induced DNA Damage

doi: 10.3390/nano11081930

Figure Lengend Snippet: Characterization of the NPs used in this study.

Article Snippet: Titanium dioxide nanoparticles (TiO 2 NPs) (rutile:anatase 15:85) (CAS No. 13463-67-7) was purchased from Degussa-Evonik (Bitterfeld, Germany).

Techniques: Zeta Potential Analyzer

Journal: Nanomaterials

Article Title: Salivary Leucocytes as In Vitro Model to Evaluate Nanoparticle-Induced DNA Damage

doi: 10.3390/nano11081930

Figure Lengend Snippet: Results of primary DNA damage induced in salivary leucocytes exposed to TiO 2 , ZnO and CeO 2 NPs. PC: positive control (50 µg/mL MMS for 3 h). Columns represent mean values and error bars indicate the standard errors. * p < 0.01, significant difference with regard to the negative control.

Article Snippet: Titanium dioxide nanoparticles (TiO 2 NPs) (rutile:anatase 15:85) (CAS No. 13463-67-7) was purchased from Degussa-Evonik (Bitterfeld, Germany).

Techniques: Positive Control, Negative Control

Journal: Nanomaterials

Article Title: Salivary Leucocytes as In Vitro Model to Evaluate Nanoparticle-Induced DNA Damage

doi: 10.3390/nano11081930

Figure Lengend Snippet: Results of oxidative DNA damage induced in salivary leucocytes exposed to TiO 2 , ZnO and CeO 2 NPs. PC: positive control (335 µg/mL KBrO 3 for 1 h). Columns represent mean values and error bars indicate the standard errors. * p < 0.01, significant difference with regard to the control.

Article Snippet: Titanium dioxide nanoparticles (TiO 2 NPs) (rutile:anatase 15:85) (CAS No. 13463-67-7) was purchased from Degussa-Evonik (Bitterfeld, Germany).

Techniques: Positive Control, Control

Journal: Toxicology Reports

Article Title: Autophagy regulates the Wnt/GSK3β/β-catenin/cyclin D1 pathway in mesenchymal stem cells (MSCs) exposed to titanium dioxide nanoparticles (TiO 2 NPs)

doi: 10.1016/j.toxrep.2020.08.020

Figure Lengend Snippet: TiO 2 NP characteristics. (A) Diameter of TiO 2 NPs is 17.81 ± 1.86 nm. (B) Zeta potential of TiO 2 NPs is -11.1 mV. (C) XRD pattern of TiO 2 NPs show a mixture of 87 % anatase and 13 % rutile phases of titanium dioxide.

Article Snippet: Titanium dioxide nanoparticles (TiO 2 NPs), PBS powder, trypan blue, autophagy inhibitor 3-methyladenine (3MA), β-catenin mRNA target sequences and lipofectamine 2000 reagent were purchased from Sigma-Aldrich (St. Louis, USA). l -glutamine, penicillin, streptomycin, and α minimum essential medium (α-MEM) were purchased from Invitrogen, CA, USA.

Techniques:

Journal: Toxicology Reports

Article Title: Autophagy regulates the Wnt/GSK3β/β-catenin/cyclin D1 pathway in mesenchymal stem cells (MSCs) exposed to titanium dioxide nanoparticles (TiO 2 NPs)

doi: 10.1016/j.toxrep.2020.08.020

Figure Lengend Snippet: Effect of TiO 2 NPs on viability and autophagy in MSCs. (A) TiO 2 NPs at 50 and 100 μg/mL reduced cell viability by approximately 7 and 15 %, respectively. (B) TiO 2 NPs at 50 and 100 μg/mL increased LC3-II/LC3/I ratio by approximately 118 % and 263 %, respectively. TiO 2 NPs at 5 and 50 μg/mL did not markedly affect these parameters. * is significantly different from control.

Article Snippet: Titanium dioxide nanoparticles (TiO 2 NPs), PBS powder, trypan blue, autophagy inhibitor 3-methyladenine (3MA), β-catenin mRNA target sequences and lipofectamine 2000 reagent were purchased from Sigma-Aldrich (St. Louis, USA). l -glutamine, penicillin, streptomycin, and α minimum essential medium (α-MEM) were purchased from Invitrogen, CA, USA.

Techniques:

Journal: Toxicology Reports

Article Title: Autophagy regulates the Wnt/GSK3β/β-catenin/cyclin D1 pathway in mesenchymal stem cells (MSCs) exposed to titanium dioxide nanoparticles (TiO 2 NPs)

doi: 10.1016/j.toxrep.2020.08.020

Figure Lengend Snippet: Effect of 50 and 100 μg/mL TiO 2 NPs on the Wnt/ GSK3β/β-catenin pathway cyclin D1. Increase in Wnt, β-catenin, and cyclin D1 protein expression levels were increase by TiO 2 NPs in a dose-dependent manner. Phosphorylation of GSK3β was decreased by TiO 2 NPs dose-dependently. * is significantly different from control.

Article Snippet: Titanium dioxide nanoparticles (TiO 2 NPs), PBS powder, trypan blue, autophagy inhibitor 3-methyladenine (3MA), β-catenin mRNA target sequences and lipofectamine 2000 reagent were purchased from Sigma-Aldrich (St. Louis, USA). l -glutamine, penicillin, streptomycin, and α minimum essential medium (α-MEM) were purchased from Invitrogen, CA, USA.

Techniques: Expressing

Journal: Toxicology Reports

Article Title: Autophagy regulates the Wnt/GSK3β/β-catenin/cyclin D1 pathway in mesenchymal stem cells (MSCs) exposed to titanium dioxide nanoparticles (TiO 2 NPs)

doi: 10.1016/j.toxrep.2020.08.020

Figure Lengend Snippet: Effect of autophagy inhibition 3MA on the Wnt/ GSK3β/β-catenin pathway and cyclin D1 regulations following exposure to 50 and 100 μg/mL TiO 2 NPs. (A) TiO 2 NPs increase the protein expression level of Wnt while 3MA reduced the changes significantly. (B) Phosphorylation level of GSK3β was decreased by TiO 2 NPs, and autophagy inhibition reversed the changes significantly. (C) Increase in β-catenin protein expression level induced by TiO 2 NPs was reverted markedly by 3MA. (D) TiO 2 NP-induced increase in cyclin D1 protein expression level was significantly reduced by autophagy inhibition. * is significantly different from control. # is significantly different from the group with the same dose of TiO 2 NP.

Article Snippet: Titanium dioxide nanoparticles (TiO 2 NPs), PBS powder, trypan blue, autophagy inhibitor 3-methyladenine (3MA), β-catenin mRNA target sequences and lipofectamine 2000 reagent were purchased from Sigma-Aldrich (St. Louis, USA). l -glutamine, penicillin, streptomycin, and α minimum essential medium (α-MEM) were purchased from Invitrogen, CA, USA.

Techniques: Inhibition, Expressing

Journal: Toxicology Reports

Article Title: Autophagy regulates the Wnt/GSK3β/β-catenin/cyclin D1 pathway in mesenchymal stem cells (MSCs) exposed to titanium dioxide nanoparticles (TiO 2 NPs)

doi: 10.1016/j.toxrep.2020.08.020

Figure Lengend Snippet: Effect of β-catenin-siRNA on cyclin D1 following exposure to 50 and100 μg/mL TiO 2 NPs. (A) β-catenin-siRNA reduces β-catenin protein expression levels to lower than that in the control group. (B) β-catenin-siRNA significantly decreases cyclin D1 protein expression level. β-catenin did not lower cyclin D1 protein expression level back to control level. * is significantly different from control. # is significantly different from the group with the same dose of TiO 2 NP.

Article Snippet: Titanium dioxide nanoparticles (TiO 2 NPs), PBS powder, trypan blue, autophagy inhibitor 3-methyladenine (3MA), β-catenin mRNA target sequences and lipofectamine 2000 reagent were purchased from Sigma-Aldrich (St. Louis, USA). l -glutamine, penicillin, streptomycin, and α minimum essential medium (α-MEM) were purchased from Invitrogen, CA, USA.

Techniques: Expressing